Complex_086

Description : RSC complex

Functional Grouping : Chromosome dynamics

Subcellular localisation : Chromatin

Comments : There are several forms of RSC. Thus, the inclusion of Rsc1 and Rsc2 appearsmutually exclusive and some forms lack Rsc3 and Rsc30.An EM study of this ring-shaped complex has been published [12368485]. Arp7 and Arp9 have an actin fold. Rsc4 contains 2 bromodomains (55-160, 184-294). Rsc3 contains a Gal4 domain (8-53). Rsc1 contains 2 bromodomains (6-114, 234-344) and a BAH domain (368-486). Sth1 contains a DEXDc domain (466-658), a helicase domain (821-905) and a bromodomain (1251-1359). Rsc30 contains a Gal4 domain (9-54). Rsc8 contains a SANT domain (311-360). Rsc2 contains 2 bromodomains (14-122, 274-384) and a BAH domain (408-526). Isw1 contains a DEXDc domain (192-384), a HELICc domain (532-616) and two SANT domains (883-933, 988-1052).

Affinity purifications :

EM rank : 3

Bioinformatics
Component	Length	Status	Pfam Doms	COGs	PDB match	Modellable	PFAM Ints	SCOP Ints
ISW1	1129	new	SNF2_N helicase_C	x	Good	Domain	no	yes
RSC6	483	known		x	-	-	no	no
RSC3	885	known	Zn_clus	x	Good	Domain	no	no
RSC8	557	missing	SWIRM ZZ myb_DNA-binding	x	Marginal	Domain	no	yes
RSC1	928	missing	BAH bromodomain	x	Good	Domain	yes	yes
RSC30	883	missing		x	Good	Domain	no	no
STH1	1359	missing	SNF2_N bromodomain helicase_C	x	Good	Domain	no	yes
RSC4	625	known	bromodomain	x	Good	Domain	no	yes
RSC58	502	missing		x	-	-	no	no
SFH1	426	missing	SNF5	x	-	-	no	no
RSC2	889	missing	BAH bromodomain	x	Good	Domain	yes	yes
RSC9	581	missing		x	-	-	no	no
ARP9	467	missing	actin	x	Good	All	yes	yes
NPL6	435	new		x	-	-	no	no
ARP7	477	missing	actin	x	Good	All	yes	yes

Potential Cross-talk
Confidence 1(low)->7(high)	Complex1	Complex2	Comp1	Comp2	Exp	PFAM1	PFAM2	Template	Zscore	PID1	E-val1	Begin1	End1	PID2	E-val2	Begin2	End2
5	086	137	ISW1	ECM16	yes	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
3	086	074	ARP9	ACT1	no	actin	actin	1o1gzx	4.72	24	0.008	43	170	87	0.0	1	370
3	086	153	ARP9	ARP2	no	actin	actin	1o1gzx	3.22	24	0.008	43	170	46	e-100	1	382
3	086	153	ARP9	ARP3	no	actin	actin	1ijjba	3.66	24	0.006	43	170	37	2e-61	5	351
3	086	074	ARP7	ACT1	no	actin	actin	1o1gzx	5.50	25	1e-13	7	185	87	0.0	1	370
3	086	153	ARP7	ARC35	no	actin	p34-Arc	1k8kad	3.96	24	0.002	8	178	38	6e-34	4	260
2	086	074	ARP7	ARP4	no	actin	actin	1o1gzx	0.82	25	1e-13	7	185	29	2e-18	2	188
2	086	153	ARP7	ARP2	no	actin	actin	1o1gzx	2.15	25	1e-13	7	185	46	e-100	1	382
2	086	153	ARP7	ARP3	no	actin	actin	1ijjab	-0.7	26	4e-14	7	185	37	2e-61	5	351
1	086	131	ISW1	RAD3	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
1	086	137	STH1	ECM16	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
1	086	251	STH1	SKI2	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
1	086	150	RSC2	ORC1	no	BAH	BAH	x	x	x	x	x	x	x	x	x	x
1	086	074	ARP9	ARP4	no	actin	actin	1o1gzx	0	24	0.008	43	170	29	2e-18	2	188
1	086	153	ARP9	ARC15	no	actin	p16_Arc	x	x	x	x	x	x	x	x	x	x
1	086	153	ARP9	ARC19	no	actin	ARPC4	x	x	x	x	x	x	x	x	x	x
1	086	153	ARP9	ARC18	no	actin	P21-Arc	x	x	x	x	x	x	x	x	x	x
1	086	131	ISW1	SSL2	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
1	086	153	ARP9	ARC35	no	actin	p34-Arc	x	x	x	x	x	x	x	x	x	x
1	086	153	ARP7	ARC15	no	actin	p16_Arc	x	x	x	x	x	x	x	x	x	x
1	086	153	ARP7	ARC19	no	actin	ARPC4	1k8kaf	2.02	24	0.002	8	178	69	6e-52	3	168
1	086	153	ARP7	ARC18	no	actin	P21-Arc	1k8kae	0	24	0.002	8	178	45	1e-40	1	177
1	086	251	ISW1	SKI2	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
1	086	105	RSC8	SWI3	no	myb_DNA-binding	myb_DNA-binding	x	x	x	x	x	x	x	x	x	x
1	086	136	RSC8	SWI8	no	myb_DNA-binding	myb_DNA-binding	x	x	x	x	x	x	x	x	x	x
1	086	150	RSC1	ORC1	no	BAH	BAH	x	x	x	x	x	x	x	x	x	x
1	086	131	STH1	RAD3	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x
1	086	131	STH1	SSL2	no	helicase_C	DEAD	x	x	x	x	x	x	x	x	x	x

Patrick Aloy